body fluid cytology; conventional cytology; cytopreparatory technique; normal saline; rehydration method. Two fixation methods can affect the quality of cytological staining derived from fine-needle aspiration biopsy, but both fixation methods have drawbacks. Federal government websites often end in .gov or .mil. Cytomorphologic comparison between rehydrated air-dried and conventional wet-fixed pap smears. and transmitted securely. Acta Cytol. Pap staining is regarded as the ringleader of stain for assessment of chromatin pattern in cervical smears with a prerequisite of immediate fixation in 95% ethanol. However, staining can sometimes hinder wet-mount evaluation by introducing. 2021 Jul;16(7):1149-1165. doi: 10.1016/j.jtho.2021.03.013. Both images 100 oil objective with aqueous Romanowsky quick stain. 2016;60(1):3944. 2018 Oct-Dec;35(4):199-203. doi: 10.4103/JOC.JOC_186_17. (B) Sample from a dog with a suspected urinary tract infection. MGG stain is one of the Romanowsky stains, which is characterized by Romanowsky effects, i.e., polychromatic effects caused by the mixture of methylene blue, methylene azur, and eosin in the staining solution [5]. In conventional body fluid cytology, air-dried smears covered with normal saline (rehydration method) before wet fixation is simple, practical and effective to minimize the cell loss, enlarge and preserve cytologic features of malignant cells with negligible extra load and cost during cytopreparation. Staining. Google Scholar. 10 0 obj HTP Flashcards | Quizlet Rehydration of Air-Dried Smears versus Wet Fixation: A Cross-Sectional Study. Aim: (A) Sample from a dog presented for a routine wellness examination. 6 0 obj Bookshelf Cytomorphologic comparison between rehydrated air-dried and %PDF-1.5 The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes. ONeil E, Horney B, Burton S, et al. 1904;37:30811. Careers. It is removed before the immunostaining procedure in two changes of 95% methanol and further fixation is carried out as required. 2013;57(4):364-8. doi: 10.1159/000351362. Ribbing method slide preparation technique. Are you sure you want to quit the test?All your progress will be lost. Wolters Kluwer Health, Inc. and/or its subsidiaries. Rehydration of air-dried smears with normal saline. To date, we have not come across an antigen that cannot be immunostained in this material which comprises lymphomas, neural tumours, carcinomas etc., and we have found that the routine antibody dilutions are applicable. For each specimen, two scrape cytology smears were wet fixed in 95% ethanol, and 12 smears were air dried without fixation. The technical storage or access that is used exclusively for statistical purposes. CAS The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
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