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10.2B). Figure 10.1. protein or carbohydrate concentrations, etc) to pass this checkpoint. Means for Classification: Saccharomyces cerevisiae is in the fungi kingdom. J = \frac{{r \cdot {\rho _x}}}{{\left( {{{{S_{TS}}}/{{V_{TV}}}}} \right)}} With the aid of FC, it becomes possible to semi-quantitatively estimate the morphological variation of the individual yeast cells. 5). This is possible by plunge-freezing of an optically transparent sample sandwich, so that the temporal resolution is only determined by the transfer speed from the fluorescence microscope to the freezing device. The two-phase regression analysis has revealed, that almost 3-folds variation of cellular granularity within 18C < T < 40C is not accompanied by the same degree of variability in total approximated cell volume of an averaged cell in population VTV(r = 0.807, R2 = 0.652, P = 0.008), whereas at T < 15C, the change in one parameter causes adequate change in the other (r = 0.999, R2 = 0.999, P = 0.0006). The experimental part of the research has been carried out in Institute of Biochemical Engineering (IBVT, University of Stuttgart, Germany) and has been funded by the transnational research initiative Systems Biology of Microorganisms (SysMO) within network MOSES: MicroOrganism Systems Biology: Energy and Saccharomyces cerevisiae [http://www.sysmo.net]. Flame an inoculating loop and allow it to cool. Datasets at Figs 4 and 5 were fit to one-phase exponential decay function to reveal the asymptotes. As is true of CKII from other organisms, the native holoenzyme is tetrameric. Next, heat fix the slide by placing it on the slide warmer for 5 minutes. checkpoints) that ensure proper division of the cell (Porro etal.2009). To better understand this mold in transition, a team of French scientists recently sequenced and analyzed the entire genome of G. candidum. Averaged diameter of budding cells in S/G2/M growth phases (Fig. maximum specific growth rate, biomass yield, specific rate of glucose consumption) were additionally determined from the same cultures (Table1; as exemplified in Fig.