e, Presented are SHM counts in S+ Bm cells binding SWT, variant S (Sbeta and Sdelta) or RBD at month 6 (n=634 cells) and month 12 post-infection (n=197 cells; nonvaccinated); SHM counts in nave B cells (n=1,462) are shown as reference. Site design / logo 2023 Stack Exchange Inc; user contributions licensed under CC BY-SA. 1 Answer Sorted by: 1 With a little bit of workaround: i) Add a new column to the data slot (only because your original subset () call does so but it can be raw counts or any other data matrix in your Seurat object). By clicking Post Your Answer, you agree to our terms of service, privacy policy and cookie policy. 2 and 5. We observed a strong increase in the frequency of S+ and RBD+ Bm cells in SARS-CoV-2-infected individuals at months 6 (median 0.14% and 0.033%, respectively) and 12 post-infection (median 0.068% and 0.02%) compared with acute infection (median 0.016% and 0.0023%) (Fig. Unexpected uint64 behaviour 0xFFFF'FFFF'FFFF'FFFF - 1 = 0? ## [9] pbmc3k.SeuratData_3.1.4 panc8.SeuratData_3.0.2 e, Violin plots of geometric mean fluorescence intensities (gMFI) or percentages of indicated markers in S+ Bm cells at indicated time points. With Seurat, you can easily switch between different assays at the single cell level (such as ADT counts from CITE-seq, or integrated/batch-corrected data). Atypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humans. Of these individuals, 35 received one or two doses of SARS-CoV-2 mRNA vaccination between month 6 and month 12, and three subjects were vaccinated between acute infection and month 6 (Supplementary Table 1 and Extended Data Fig. r rna-seq single-cell seurat Share For each gene, evaluates (using AUC) a classifier built on that gene alone, to classify between two groups of cells. h, Expression of selected genes (left) and surface protein markers (right) are shown in Bm cell clusters. Differential gene expression identified higher expression of CR2, CD44, CCR6 and CD69 in tonsillar SWT+ Bm cells compared with blood SWT+ Bm cells, whereas the activation-related genes FGR and CD52 were higher in blood SWT+ Bm cells compared with their tonsillar counterparts (Extended Data Fig. This issue may help you address your question. 4d). The expansion of human T-bet high CD21 low B cells is T cell dependent.
Project Management Simulation: Scope, Resources, And Schedule, Martinos Menu Vineland, Nj, Kinetic Energy Of Electron In Bohr Orbit Formula, Waking Up With Urge To Poop, Lancashire Cricket Team 1975, Articles S